Modified colorimetric ultramicro method for estimating Nefa in serum

Modified colorimetric ultramicro method for estimating Nefa in serum.

A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 mul samples; it is thus suitable for work on newborn babies and small laborato...

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.

An ultramicro colorimetric method for determination of total and direct serum bilirubin.

A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.

Modified colorimetric method for procainamide in plasma.

We describe a modified colorimetric method for determining procainamide in plasma by use of a diazotization and coupling reaction with N-(1-naphthyl)-ethylenediamine dihydrochloride (Marshall's reagent). The sensitivity of the assay is increased twofold over the originally reported colorimetric method, and the lower limit of detection for the assay of a 1.0-ml plasma sample has been decreased t...

Ultramicro method for serum bilirubin by diazo blue reaction.

ROPER CLINICAL MANAGEMENT of infants with erythroblastosis fetalis necessitates the determination of total serum bilirubin. Of the many modifications of the original diazo reaction on blood serum (1) a micromethod using capillary blood is preferable. One tenth miffiliter of serum was used by Davidson et al. (2) in their modification of the Ernst and F#{246}rster (3) method and by With (4, 5) in...